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The 2nd reaction cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis. As attracted Fig - https://padlet.com/julieanndesalorenz30606/olis-clarity-srqqvp7768okh664. 9, the reaction may likewise include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As shown in Fig. 9, this might be due to the fact that the H4B radical function other as an electron sink in a later point in the response, so as to permit NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.